DUSP13B/TMDP inhibits stress-activated MAPKs and suppresses AP-1-dependent gene expression

作者：Katagiri Chiaki; Masuda Kouhei; Nomura Miyuki; Tanoue Kan; Fujita Shoko; Yamashita Yoji; Katakura Ryuichi; Shiiba Ken ichi; Nomura Eiki; Sato Masami; Tanuma Nobuhiro; Shima Hiroshi^*

来源：Molecular and Cellular Biochemistry, 2011, 352(1-2): 155-162.

DOI：10.1007/s11010-011-0749-x

• Atypical dual-specificity phosphatase
• DUSP13
• TMDP
• MAPK
• AP-1
• Spermatogenesis

The dual-specificity phosphatase (DUSP) 13 gene encodes two atypical DUSPs, DUSP13B/TMDP and DUSP13A/MDSP using alternative exons. DUSP13B protein is most highly expressed in testis, particularly in spermatocytes and round spermatids of the seminiferous tubules, while that of DUSP13A is restricted to skeletal muscle. Here, we show that DUSP13B inactivated MAPK activation in the order of selectivity, JNK = p38 > ERK in cells, while DUSP13A did not show MAPK phosphatase activity. Reporter gene analysis showed that DUSP13B had significant inhibitory effect on AP-1-dependent gene expression, but DUSP13A did not. To our knowledge, DUSP13B is the first identified testis-specific phosphatase that inhibits stress-activated MAPKs. These data suggest an important role for DUSP13B in protection from external stress during spermatogenesis.

• 出版日期2011-6
• 单位河北医科大学