科研之友
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摘要
Objectives: Lysyl oxidase (LOX) always promotes cancer cell metastasis. This study aimed to investigate the influence of LOX G473A on ovarian cancer cells function and the related cell signaling pathways. Methods: Expression of LOX G473A in 3 ovarian cancer cell lines HO-8910 and A2780 as well as the siRNA-LOX G473A transfected SKOV3 cells were detected by western blotting analysis. Migration and invasion as well as mitosis ability of transfected cells were detected using in vitro transwell assay and flow cytometry, respectively. Cytokines related to cell migration, invasion and metastasis were detected to investigate the influence of siRNA-LOX G473A on their expression. Results: LOX G473A showed the highest expression level in SKOV3 cell line compared with the other two. SiRNA-LOX G473A significantly reduced the expression of LOX G473A protein as well as cell migration, invasion and mitosis abilities. Moreover, cytokines associated with cell migration, invasion and metastasis were dysregulated by siRNA-LOX G473A. Conclusions: LOX G473A was a crucial factor modulating cell migration, invasion and metastasis of ovarian cancer cell lines. The facts of LOX G473A silence could reduce cell migration, invasion and metastasis abilities demonstrated that LOX G473A might be served as a potential therapeutic target for ovarian cancer cells.
