Adhesion and immunomodulatory effects of Bifidobacterium lactis HN019 on intestinal epithelial cells INT-407

作者：Liu, Chang; Zhang, Zhuo Yang; Dong, Ke; Guo, Xiao Kui^*

来源：World Journal of Gastroenterology, 2010, 16(18): 2283-2290.

DOI：10.3748/wjg.v16.i18.2283

• Probiotics
• Intestinal epithelium
• Enteropathogen
• Immunomodulation
• Adhesion

AIM: To elucidate the adherence and immunomodulatory properties of a probiotic strain Bifidobacterium lactis (B. lactis) HN019.
METHODS: Adhesion assays of B. lactis HN019 and Salmonella typhimurium (S. typhimurium) ATCC 14028 to INT-407 cells were carried out by detecting copies of species-specific genes with real-time polymerase chain reaction. Morphological study was further conducted by transmission electron microscopy. Interleukin-1 beta (IL-1 beta), interleukin-8, and tumor necrosis factor-alpha (TNF-alpha) gene expression were assessed while enzyme linked immunosorbent assay was used to detect IL-8 protein secretion.
RESULTS: The attachment of S. typhimurium ATCC 14028 to INT407 intestinal epithelial cells was inhibited significantly by B. lactis HN019. B. lactis HN019 could be internalized into the INT-407 cells and attenuated IL-8 mRNA level at both baseline and S. typhimurium-induced pro-inflammatory responses. IL-8 secretion was reduced while IL-1 beta and TNF-alpha mRNA expression level remained unchanged at baseline after treated with B. lactis HN019.
CONCLUSION: B. lactis HN019 does not up-regulate the intestinal epithelium expressed pro-inflammatory cytokine, it showed the potential to protect enterocytes from an acute inflammatory response induced by enteropathogen.

• 出版日期2010-5-14
• 单位上海交通大学; 河北医科大学