Anti-inflammatory activities of Dangyuja (Citrus grandis Osbeck) in concanavalin A stimulated murine splenocytes and 12-O-tetradecanoylphorbol-13-acetate-induced murine skin edema

作者：Herath Kalahe Hewage Iresha Nadeeka Madushani; Bing So Jin; Cho Jinhee; Kim Areum; Shin Sumin; Kim Gi Ok; Lee Jong chul; Jee Youngheun^*

来源：Biomedicine & Pharmacotherapy, 2016, 83: 1353-1364.

DOI：10.1016/j.biopha.2016.08.042

• Skin inflammation
• Anti-inflammatory cytokines
• iNOS
• COX-2
• MPO activity

Dangyuja (Citrus grandis Osbeck), a citrus cultivated in southern Korea, has been used in traditional medicine for its anti-inflammatory effect. In this study, we investigated the anti-inflammatory potential of extract of Citrus grandis Osbeck (ECGO). In in vitro assays, ECGO treatment of concanavalin A (10 mu g/ml, for 24 h) stimulated splenocytes showed significant reduction in CD44/CD62L(+) T cell population and a marked decrease in the production of inflammatory cytokines IL-2, IFN-gamma and IL-4. Interestingly, in vivo assays of ECGO topical treatment (100 mu g/20 mu l/ear) significantly mitigated the TPA (4 mu g/20 mu l/ear) induced edema induction and Myeloperoxidase activity. Anti-inflammatory potential of ECGO were further evidenced through its potent decrease in expression of inducible nitric oxide, cyclooxygenase-2, IL-1 beta and TNF-alpha and suppressed homing of CD3(+) T cells and F4/80(+) macrophages to site of inflammation. This study emphasizes the possibility of developing ECGO as an alternative natural topical agent to combat inflammatory diseases.

• 出版日期2016-10