alpha Pix enhances mutant huntingtin aggregation

作者：Eriguchi Makoto; Mizuta Haruo^*; Luo Shouqing; Kuroda Yasuo; Hara Hideo; Rubinsztein David C

来源：Journal of the Neurological Sciences, 2010, 290(1-2): 80-85.

DOI：10.1016/j.jns.2009.11.003

• alpha Pix
• Pix
• Huntingtin
• Polyglutamine
• Neurodegenerative disease
• Aggregation

Huntington's disease is caused by polyglutamine-expanded mutant huntingtin (muhtt), an aggregation-prone protein. We identified the Pak-interacting exchange factor (alpha Pix/Cool2) as a novel huntingtin (htt) interacting protein, after screening actin-cytoskeleton organization-related factors. Using immunoprecipitation experiments, we show that alpha Pix binds to both the N-terminal of wild-type htt (wthtt) and mutant htt (muthtt). Colocalization studies revealed that (x Pix accumulates in muthtt aggregates. Deletion analysis suggested that the dbl homology (DH) and pleckstrin homology (PH) domains of alpha Pix are required for its interaction with htt. Overexpression of ot Pix enhanced muthtt aggregation by inducing SDS-soluble muthtt-muthtt interactions. Conversely, knocking down ot Pix attenuated muhtt aggregation. These findings suggest that ot Pix plays an important role in muthtt aggregation.

• 出版日期2010-3-15
• 单位河北医科大学