A new conjugated poly(pyridinium salt) derived from phenanthridine diamine: its synthesis, optical properties and interaction with calf thymus DNA

作者：Sun Yujiao; Wang Jing^*; Jin Lu; Chang Ying; Duan Jingjing; Lu Yan

来源：Polymer Journal, 2015, 47(11): 753-759.

DOI：10.1038/pj.2015.62

A new conjugated poly(pyridinium salt) derivative (P1) containing heterocyclic phenanthridine moieties in the main chain with tosylate counterions was prepared via a ring-transmutation polymerization reaction. Its chemical structure was established using NMR and FTIR spectra. Its number-average molecular weight (M-n) and polydispersity were 14 430 and 1.456, respectively, as determined by gel permeation chromatography (GPC). The interaction of P1 with calf thymus DNA (ctDNA) in an ethanol-Tris-HCl buffer (2 mM, pH 7.4) (v/v= 1: 1) was investigated by ultraviolet-visible, fluorescence, circular dichroism spectroscopy, and dynamic light scattering (DLS) measurements. The electrostatic attraction and intercalation interaction between P1 and ctDNA resulted in a significant fluorescence quenching of polymer P1 in the ethanol-Tris-HCl buffer, and the ratio of fluorescence intensity at 523 nm in the absence of ctDNA (F-0) to that in the presence of ctDNA (F-i) was linearly proportional to the concentration of ctDNA within the range of 0-6.0 mu M. Therefore, the conjugated polyelectrolyte is expected to be used as a new fluorescent probe for the label-free detection of DNA.

• 出版日期2015-11
• 单位天津理工大学