科研之友
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摘要
Purpose: To investigate the antioxidant and anti-inflammatory effects of Ulmus davidiana extract (UDE) in lipopolysaccharide (LPS)-stimulated BV-2 cells. Methods: Antioxidant activity was measured using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. BV-2 cells were stimulated with LPS to study protein expression and production of inflammatory mediators, and determined by Western blot analysis. Results: UDE significantly inhibited DPPH-generated free radicals showing maximum inhibition at 40 mu g/mL (p < 0.001). UDE alone did not exhibit any signs of cytotoxicity towards BV-2 cells up to 100 mu g/mL concentration. The LPS-induced increase in the production of nitric oxide was concentration-dependently suppressed with half-maximal concentration (IC50) of 67.4 mu g/mL of UDE (p < 0.05 at 10 mu g/mL, p < 0.01 at 20 mu g/mL and p < 0.001 at 40 mu g/mL, respectively). UDE also inhibited dose-dependently the LPS-induced increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions with IC50 of 52.3 ug/mL. Furthermore, the production of pro-inflammatory cytokines, via tumor necrosis factor-alpha by LPS-stimulation in BV2 murine cells was inhibited dose-dependently with IC50 of 85.1 ug/mL by UDE pretreatment. Mechanistic studies revealed that UDE acts by regulation of nuclear factor kappa-B signaling pathway in LPS-stimulated BV-2 cells. Conclusion: This study shows, for the first time, that UDE possesses antioxidant and anti-inflammatory effects and can be developed as a potential therapeutic agent for ameliorating macrophage-mediated inflammation.
- 出版日期2016-1
