In vitro evaluation of putative virulence attributes of oral isolates of Candida spp. Obtained from elderly healthy individuals

作者：Furlaneto Maia Luciana; Specian Ana Flavia; Bizerra Fernando Cesar; de Oliveira Marcelo Tempesta; Furlaneto Marcia Cristina

来源：Mycopathologia, 2008, 166(4): 209-217.

DOI：10.1007/s11046-008-9139-7

• Candida spp
• virulence factors
• healthy individuals

Identification of Candida isolates obtained from oral cavity of elderly healthy individuals revealed the predominance of non-albicans Candida species (88.9%) compared to Candida albicans (11%). CHROMagar Candida differential medium and PCR revealed the presence of Candida tropicalis (33.3%), Candida glabrata (27.8%), and Candida krusei (16.7%). We investigated the presence of virulence attributes in a total of 18 isolates, including acid protease and phospholipase production, hemolytic activity, and biofilm production. Extracellular protease was found in five isolates (27.8%) whereas extracellular phospholipase was found in three isolates (17%). All isolates showed hemolytic activity. About 56% of the isolates were weakly positive for biofilm formation (score +) whereas a minority (5.6%) of them showed strong biofilm formation (score 4+). Susceptibility in vitro of the isolates to fluconazole was carried out by microdilution method. Fluconazole showed a strong inhibition against most buccal isolates. The resistant isolates were 2 C. tropicalis, 2 C. glabrata, and 1 C. krusei.

• 出版日期2008-10