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摘要
A simple method, based on dispersive liquid-liquid microextraction coupled with ionic liquid, (IL-DLLME) was applied for the first time to simultaneously detect formaldehyde in air, water, and soil samples. The ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate ([C8MIM][PF6]) was utilized as the extraction solvent, and formaldehyde was detected by IL-DLLME combined with high-performance liquid chromatography and ultraviolet detection (HPLC-UV). The versatile derivatization agent, 2,4-dinitrophenylhydrazine (DNPH), was employed in IL-DLLME to react with formaldehyde before extraction. Aided by the disperser solvent (methanol), [C8MIM][PF6] was quickly dispersed into the sample solution to extract 2,4-dinitrophenylhydrazone (HCHO-DNPH). Parameters affecting extraction efficiency, including extraction solvent types and volumes, disperser solvent types and volumes, and formic acid volumes and salt additions, were investigated and optimized by a step-by-step procedure. The derivatization conditions of formaldehyde were optimized using orthogonal array optimization (OAD). Under the optimized conditions, the enrichment factor was 355 and the calibration curve with a correlation coefficient of 0.9996. The limit of detection (LOD) was 0.01-0.09ngmL(-1). The proposed method was then applied to the analysis of formaldehyde in air, water, and soil samples, and the recoveries of the spiked samples were 71.6-103.5% and 76.8-102.7% at concentrations of 50ngmL(-1) and 100ngmL(-1), respectively.
- 出版日期2014-4-3
- 单位中国农业大学
