Alignment of human vascular smooth muscle cells on parallel electrospun synthetic elastin fibers

作者：Nivison Smith Lisa; Weiss Anthony S^*

来源：Journal of Biomedical Materials Research Part A, 2012, 100A(1): 155-161.

DOI：10.1002/jbm.a.33255

• smooth muscle cells
• alignment
• parallel fibers
• elastin
• electrospinning

We generated parallel elastic fibers from synthetic elastin (SE) as a model of the arterial media and assessed the alignment of smooth muscle cells (SMCs). SE utilized crosslinked electrospun human tropoelastin to form aligned fibers that mimicked the topography and elastin-rich content of the medial extracellular matrix. Bundled parallel fibers were anisotropically more elastic than randomly arranged scaffolds (111 +/- 25 kPa vs. 265 +/- 17 kPa) in the direction of the fibers. Aligned and random fiber scaffolds each supported SMC growth. Following attachment, SMCs proliferated longitudinally on the parallel fibers and expressed native alpha-smooth muscle actin.

• 出版日期2012-1