Novel analytical procedure using a combination of hollow fiber supported liquid membrane and dispersive liquid-liquid microextraction for the determination of aflatoxins in soybean juice by high performance liquid chromatography - Fluorescence detector

作者：Simao Vanessa; Merib Josias; Dias Adriana N; Carasek Eduardo^*

来源：Food Chemistry, 2016, 196: 292-300.

DOI：10.1016/j.foodchem.2015.09.018

• Hollow fiber supported liquid membrane
• Dispersive liquid-liquid microextraction
• Soybean juice
• Aflatoxins
• Sample preparation

This study describes a combination between hollow fiber membrane and dispersive liquid-liquid microextraction for determination of aflatoxins in soybean juice by HPLC. The main advantage of this approach is the use of non-chlorinated solvent and small amounts of organic solvents. The optimum extraction conditions were 1-octanol as immobilized solvent; toluene and acetone at 1:5 ratio as extraction and disperser solvents (100 mu L), NaCl at 2% of the sample volume and extraction time of 60 min. The optimal condition for the liquid desorption was 150 mu L acetonitrile:water (50:50 v/v) and desorption time of 20 min. The linear range varied from 0.03 to 21 mu g L-1, with R-2 coefficients ranging from 0.9940 to 0.9995. The limits of detection and quantification ranged from 0.01 mu g L-1 to 0.03 mu g L-1 and from 0.03 mu g L-1 to 0.1 mu g L-1, respectively. Recovery tests ranged from 72% to 117% and accuracy between 12% and 18%.

• 出版日期2016-4-1