Diagnostic efficiency of KISS1 mRNA combined with CA-125 in detection of epithelial ovarian cancer

作者：Chen, Liping; Liu, Manhua; Lin, Weiwei; Ji, Jinlong; Xiao, Jing; Liu, Hongbin; He, Bosheng^*

来源：International Journal of Clinical and Experimental Medicine, 2017, 10(4): 6648-6655.

• KISS1 gene
• CA-125
• epithelial ovarian cancer
• ROC curve
• diagnosis

The aim of this study was to evaluate the sensitivity and specificity of KISS1 mRNA combined with CA-125 in detection of the epithelial ovarian cancer (EOC). The tissue and blood KISS1 mRNA expression levels and serum CA-125 level from EOC patients and control groups were measured by using RT-PCR method and chemiluminescence method. The four control groups were borderline ovarian tumor patients (control group 1), benign epithelial ovarian tumor patients (control group 2), leiomyoma of uteruspatients (control group 3) and healthy people (control group 4). The tissue or blood KISS1 mRNA and serum CA-125 concentrations were significantly higher in EOC patients than that in healthy people (P < 0.001). However, there were no significant differences in tissue or blood KISS1 mRNA and serum CA-125 among control group 2, 3 and 4. The receiver-operator characteristic (ROC) analysis showed that the area under the curves (AUC) were 0.817, 0.696 and 0.853, respectively for tissue KISS1 mRNA, blood KISS1 mRNA and serum CA 125, while there was an increase in the AUC (0.925) for serum CA-125 combined with blood KISS1. KISS1 mRNA can be used as an additional tumor biomarker for diagnosis of EOC. Multiple biomarkers assay of combined blood KISS1 and serum CA-125 is superior to single biomarker alone in detection of EOC. KISS1 may exert a synergistic or additional effect on CA-125.

• 出版日期2017
• 单位南通大学