科研之友
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摘要
A simple, rapid, and accurate micellar electrokinetic chromatographic method has been developed for determination of gastrodin and tetramethylpyrazine in three traditional Chinese preparations: Zhennaoning jiaonang, Yangxue shengfa jiaonang, and Xiaoshuan zaizao wan. Running buffer comprising 50 mM sodium tetraborate and 15 M sodium dodecylsulfate (SDS), pH 9.50, was found to be most suitable for the separation. All experiments was performed with a 47cm (40cm effective length) × 75 urn ID uncoated fused-silica capillary and UV detection at 200 nm. The linear calibration ranges were 2.5-200 μg mL-1 (R= 0.999) for gastrodin and 5.0-200 μg mL-1 (R = 0.997) for tetramethylpyrazine; the detection limits were 0.5 μg mL-1 and 0.8 μg mL-1, respectively. Recoveries of the two analytes from the samples, calculated by use of a method described in detail in the text, were between 94.21 and 104.46%. The amounts of gastrodin and tetramethylpyrazine in the preparations were easily determined within 10 min.
- 出版日期2003
- 单位清华大学
