Nanoparticle translocation across mouse alveolar epithelial cell monolayers: Species-specific mechanisms

作者：Fazlollahi Farnoosh; Kim Yong Ho; Sipos Arnold; Hamm Alvarez Sarah F; Borok Zea; Kim Kwang Jin; Crandall Edward D^*

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2013, 9(6): 786-794.

DOI：10.1016/j.nano.2013.01.007

• Epithelial transport
• Endocytosis
• Clathrin
• Dynamin
• Surface charge

Studies of polystyrene nanoparticle (PNP) trafficking across mouse alveolar epithelial cell monolayers (MAECM) show apical-to-basolateral flux of 20 and 120 nm amidine-modified PNP is similar to 65 times faster than that of 20 and 100 nm carboxylate-modified PNP, respectively. Calcium chelation with EGTA has little effect on amidine-modified PNP flux, but increases carboxylate-modified PNP flux similar to 50-fold. PNP flux is unaffected by methyl-beta-cyclodextrin, while similar to 70% decrease in amidine- (but not carboxylate-) modified PNP flux occurs across chlorpromazine- or dynasore-treated MAECM. Confocal microscopy reveals intracellular amidine- and carboxylate- modified PNP and association of amidine- (but not carboxylate-) modified PNP with clathrin heavy chain. These data indicate (1) amidine- modified PNP translocate across MAECM primarily via clathrin-mediated endocytosis and (2) physicochemical properties (e.g., surface charge) determine PNP interactions with mouse alveolar epithelium. Uptake/trafficking of nanoparticles into/across epithelial barriers is dependent on both nanoparticle physicochemical properties and (based on comparison with our prior results) specific epithelial cell type.
From the Clinical Editor: In this study of polystyrene nanoparticle trafficking across mouse alveolar epithelial cell monolayers, the authors determined that uptake/trafficking of nanoparticles into/across epithelial barriers is dependent on both nanoparticle physicochemical properties and the specific type of epithelial cells.

• 出版日期2013-8