科研之友
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摘要
The recent development and application of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays (ELISAs) of many kinds of organic compounds are reviewed herein. Multi-analyte ELISAs based on generic antibodies directly, or on specific antibodies by multi-antibody co-coating or multi-tracer co-marking are described in detail. Special attention was placed on the design and synthesis of a generic hapten, the manner of linking the hapten to a carrier protein, type and character of the antibody and format of the ELISAs. It is hoped that this comprehensive review can be considered as a useful reference for further investigation of multi-analyte simultaneous analysis by ELISAs.
