Mutations in the C-terminus of HBoV NS1 affect the function of NP1

作者：Zhang, Junmei; Bai, Yan; Zhu, Bing; Hao, Sujuan; Chen, Zhen; Wang, Hanzhong; Guan, Wuxiang^*

来源：Scientific Reports, 2017, 7(1): 7407.

DOI：10.1038/s41598-017-06513-4

• HUMAN BOCAVIRUS 1
• MINUTE VIRUS
• NONSTRUCTURAL PROTEINS
• RESPIRATORY VIRUSES
• HUMAN PARVOVIRUS
• REPLICATION
• HAIRPIN
• BINDING
• ORIGIN
• B19

Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome.

• 出版日期2017-8-7
• 单位广州市妇女儿童医疗中心; 中国科学院大学; 中国科学院武汉病毒研究所; 华中科技大学