Age dependent differences in the kinetics of Y delta T cells after influenza vaccination

作者：Stervbo Ulrik^*; Pohlmann Dominika; Baron Udo; Bozzetti Cecilia; Juerchott Karsten; Maelzer Julia Nora; Nienen Mikalai; Olek Sven; Roch Toralf; Schulz Axel Ronald; Warth Sarah; Neumann Avidan; Thiel Andreas; Gruetzkau Andreas; Babel Nina

来源：PLos One, 2017, 12(7): e0181161.

DOI：10.1371/journal.pone.0181161

Immunosenescence is a hallmark of the aging immune system and is considered the main cause of a reduced vaccine efficacy in the elderly. Although Y delta T cells can become activated by recombinant influenza hemagglutinin, their age-related immunocompetence during a virus-induced immune response has so far not been investigated. In this study we evaluate the kinetics of Y delta T cells after vaccination with the trivalent 2011/2012 northern hemisphere seasonal influenza vaccine. We applied multi-parametric flow cytometry to a cohort of 21 young (19-30 years) and 23 elderly (53-67 years) healthy individuals. Activated and proliferating Y delta T cells, as identified by CD38 and Ki67 expression, were quantified on the days 0, 3, 7, 10, 14, 17, and 21. We observed a significantly lower number of activated and proliferating Y delta T cells at baseline and following vaccination in elderly as compared to young individuals. The kinetics changes of activated Upsilon delta T cells were much stronger in the young, while corresponding changes in the elderly occurred slower. In addition, we observed an association between day 21 HAI titers of influenza A and the frequencies of Ki67(+) Y delta T cells at day 7 in the young. In conclusion, aging induces alterations of the Upsilon delta T cell response that might have negative implications for vaccination efficacy.

• 出版日期2017-7-11