Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules

作者:Levantino Matteo; Yorke Briony A; Monteiro Diana C F; Cammarata Marco; Pearson Arwen R
来源:Current Opinion in Structural Biology, 2015, 35: 41-48.
DOI:10.1016/j.sbi.2015.07.017

摘要

Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.

  • 出版日期2015-12