Alleviation of neuropathic pain by intrathecal injection of antisense oligonucleotides to p65 subunit of NF-kappa B

作者：Sun, T.; Song, W. -G.; Fu, Z. -J.; Liu, Z. -H.; Liu, Y. -M.; Yao, S. -L.^*

来源：British Journal of Anaesthesia, 2006, 97(4): 553-558.

DOI：10.1093/bja/ael209

• antisense oligodeoxynucleotides
• complications, chronic constriction injury
• NF-kappa B
• spinal cord

Background. Treatment of neuropathic pain remains a challenge. The current study investigated the therapeutic effect of intrathecal administration of NF-kappa B antisense oligodeoxynucleotides (ODNs) on mechanical allodynia and thermal hyperalgesia in a chronic constriction injury (CCI) model of rats. @@@ Methods. Lumbar intrathecal catheters were implanted in male Sprague-Dawley rats and a CCI model was established. Thermal and mechanical nociceptive thresholds were assessed with paw withdrawal latency (PWL) to radiant heat and von Frey filaments. The phosphorothioate-modified antisense ODNs to p65 subunit of NF-kappa B were administered intrathecally on each of five consecutive days post-CCI. Nuclear NF-kappa B p65 expression was assessed by western blot. @@@ Results. CCI induced mechanical allodynia and thermal hyperalgesia and significantly increased NF-kappa B p65 protein expression. Intrathecal injection of antisense ODN markedly suppressed the expression of NF-kappa B p65 protein and significantly attenuated CCI-induced mechanical allodynia and thermal hyperalgesia. @@@ Conclusion. The activation of NF-kappa B pathway may contribute to neuropathic pain in CCI rats. Suppression of NF-kappa B could be a potential new strategy for the treatment of neuropathic pain.

• 出版日期2006-10
• 单位深圳市第二人民医院; 山东省立医院; 华中科技大学