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摘要
3(II)-O-Allyl-alpha-laminaribiosyl fluoride was prepared as a key synthon for the enzymatic synthesis of beta(1 -> 3)-glucan oligosaccharides, catalyzed by a mutated beta(1 -> 3)-glucanase (E231G) from barley (Hordeum vulgare L.). A strategy was developed for enzymatic elongation of the beta(1 -> 3)-glucan chain from the reducing end, using a single glucoside acceptor. When beta-glucoside phenyl disulfide was used as the acceptor, this methodology generated laminari-oligosaccharides conjugatable at both their reducing and non-reducing ends.
- 出版日期2009
