No association between vitamin D and beta-cell autoimmunity in Finnish and Estonian children

作者：Reinert Hartwall L; Honkanen J^*; Harkonen T; Ilonen J; Simell O; Peet A; Tillmann V; Lamberg Allardt C; Virtanen S M; Knip M; Vaarala O

来源：DIABETES-METABOLISM RESEARCH AND REVIEWS, 2014, 30(8): 749-760.

DOI：10.1002/dmrr.2550

• Vitamin D
• beta-cell
• autoimmunity
• T cells
• immunoregulation

BackgroundVitamin D has immunomodulatory properties, such as regulation of FOXP3 expression and regulatory T-cell activity. Our aim was to investigate whether plasma 25-hydroxyvitamin D [25(OH)D] concentrations associate with the development of -cell autoimmunity and the transcriptional activity of FOXP3 or vitamin D3 convertase gene (CYP27B1) in CD4+ memory T cells. %26lt;br%26gt;MethodsWe studied 83 Finnish and 32 Estonian children participating in the DIABIMMUNE and DIPP studies. Twenty-nine Finnish and six Estonian children tested positive for at least one diabetes-associated autoantibody. The plasma concentrations of 25(OH)D and 1,25(OH)(2)D were analysed with an enzyme immunoassay. Gene expression of FOXP3 and CYP27B1 in the isolated CD4+ memory T cells was studied with reverse transcription quantitative polymerase chain reaction. %26lt;br%26gt;ResultsVitamin D status did not differ between subjects positive and negative for -cell autoantibodies. Finnish children had higher vitamin D status than Estonian children (p%26lt;0.001). FOXP3 expression was higher in Estonian CD4+ memory T-cell samples than in Finnish samples (p%26lt;0.01) even when including in both groups only children with serum 25(OH)D concentrations in the range of 50-80nmol/L (p%26lt;0.001). %26lt;br%26gt;ConclusionsThese findings do not support a crucial role of circulating 25(OH)D as a regulator of -cell autoimmunity or FOXP3 expression.

• 出版日期2014-11