AEG-1 participates in high glucose-induced activation of Rho kinase and epithelial mesenchymal transition in proximal tubular epithelial cells

作者：Li, Wen-Ning; Wei, Jia-Li^*; Wu, Ming; Wu, Wei; Huang, Yun; Xie, Mao-Wei; Han, Hui

来源：Asian Pacific Journal of Tropical Medicine, 2015, 8(12): 1042-1044.

DOI：10.1016/j.apjtm.2015.11.015

• Astrocyte elevated gene-1
• Epithelial-mesenchymal transition
• High glucose
• Renal tubulointerstitial fibrosis
• Rho kinase

Objective: To prove whether astrocyte elevated gene-1 (AEG-1) plays a role in high glucose-stimulated Rho kinase activation and epithelial-mesenchymal transition (EMT) in human renal tubular epithelial (HK-2) cells. Methods: The protein levels of AEG-1, alpha-smooth muscle actin, E-cadherin and MYPT1 were determined by Western blot. Results: AEG-1 protein level was upregulated in HK-2 cells stimulated with high glucose. AEG-1 siRNA downregulated Rho kinase protein expression and blocked high glucose-induced EMT. Conclusions: Our results show that AEG-1 acts a key role in high glucoseinduced activation of Rho kinase and EMT in HK-2 cells.

• 出版日期2015-12
• 单位河北医科大学