Laser-induced heating for in situ DNA replication and detection in microchannels

作者：Hung Min Sheng^*; Chen Chih Pin

来源：IET Nanobiotechnology, 2018, 12(6): 841-845.

DOI：10.1049/iet-nbt.2017.0302

• DNA
• molecular biophysics
• fluorescence
• electrophoresis
• biochemistry
• molecular configurations
• quantum dots
• laser beam applications
• biothermics
• laser-induced heating
• long DNA strand
• target DNA replication
• DNA strands
• local DNA sequences
• thermal cycles
• replicated double-stranded DNA products
• replicated local DNA sequence
• in situ DNA replication
• in situ local deoxyribonucleic acid replication
• strong avidin-biotin binding
• biosample gene sequence analysis

This study proposes a method for in situ local deoxyribonucleic acid (DNA) replication and detection in a long DNA strand through laser-induced heating and strong avidin-biotin binding. To achieve the target DNA replication, dielectrophoresis was generated to stretch and immobilise DNA strands on both ends of the electrode. Subsequently, local DNA sequences were replicated using thermal cycles generated by laser-induced heating. Replicated double-stranded DNA products were captured in situ on a solid surface and detected using the fluorescence intensity of quantum dots (Qdots). The results revealed that after six laser-induced thermal cycles, the replicated local DNA sequence could be detected by analysing the difference between Qdot fluorescent intensity before and after replication. The proposed method is expected to improve the efficiency of biosample gene sequence analysis.

• 出版日期2018-9