Multiple antigen peptide mimetics containing gp41 membrane-proximal external region elicit broad neutralizing antibodies against human immunodeficiency virus type 1 in guinea pigs

作者：Zhang, Lishuang; Miao, Liang; Gong, Xin; Zhang, Huayan; Yang, Lan; Shi, Yuhua; Kong, Wei; Jiang, Chunlai; Shan, Yaming^*

来源：Journal of Peptide Science, 2013, 19(8): 491-498.

DOI：10.1002/psc.2526

• broad neutralization
• HIV-1 vaccine
• MPER
• multiple antigen peptide mimetics

Eliciting a broadly neutralizing antibody response against the HIV-1 membrane-proximal external region (MPER) mimicking the activity of 4E10 and 2F5 monoclonal antibodies remains a major challenge. In this study, two novel tetra-branched peptide immunogens, 4E10- and 2F5-MAP4, were designed and synthesized using a MAP system. Guinea pigs were immunized with either of these two synthetic immunogens emulsified in an oil-phase adjuvant at 3-week intervals. After four immunizations, epitope-specific antibody responses were induced successfully, and moderate neutralizing activities against tier 1 (clades B, BC, AE) and tier 2 (clade C) HIV-1 pseudoviruses were detectable in unfractionated sera and purified IgGs. The synthetic gp41 membrane-proximal external region peptide mimetics, 4E10- and 2F5-MAP4, assisted by an appropriate adjuvant, are promising prophylactic vaccine candidates potentially capable of eliciting broadly neutralizing antibody responses against HIV-1.

• 出版日期2013-8
• 单位吉林大学