Neural stem cell differentiation is mediated by integrin beta 4 in vitro

作者：Su Le; Lv Xin; Xu JiPing; Yin DeLing; Zhang HaiYan; Li Yi; Zhao Jing; Zhang ShangLi; Miao JunYing^*

来源：International Journal of Biochemistry & Cell Biology, 2009, 41(4): 916-924.

DOI：10.1016/j.biocel.2008.09.001

• Integrin beta 4
• Neural stem cell
• Differentiation
• RNA interference
• Fibroblast growth factor receptor

Neural stem cells are capable of differentiating into three major neural cell types, butthe underlying molecular mechanisms remain unclear. Here, we investigated the mechanism by which integrin beta 4 modulates mouse neural stem cell differentiation in vitro. Inhibition of endogenous integrin beta 4 by RNA interference inhibited the cell differentiation and the expression of fibroblast growth factor receptor 2 but not fibroblast growth factor receptor I or fibroblast growth factor receptor 3. Overexpression of integrin beta 4 in neural stem cells promoted neural stem cell differentiation. Furthermore, integrin beta 4-induced differentiation of neural stem cells was attenuated by SU5402, the inhibitor of fibroblast growth factor receptors. Finally, we investigated the role of integrin beta 4 in neural stem cell survival: knockdown of integrin beta 4 did not affect survival or apoptosis of neural stem cells. These data provide evidence that integrin beta 4 promotes differentiation of mouse neural stem cells in vitro possibly through fibroblast growth factor receptor 2.

• 出版日期2009-4
• 单位山东大学