Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair defects post exposure to gamma ionizing radiation

作者：Garcia Sandra N; Kirtane Bhakti M; Podlutsky Andrej J; Pereira Smith Olivia M; Tominaga Kaoru^*

来源：FEBS Letters, 2007, 581(27): 5275-5281.

DOI：10.1016/j.febslet.2007.10.017

• MORF4
• NUA4
• Sin3-HDAC
• ATM
• 53BP1

MORF4-related gene on chromosome 15 (MRG15) is a core component of the NuA4/Tip60 histone acetyltransferase complex that modifies chromatin structure. We here demonstrate that Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit an impaired DNA-damage response post gamma irradiation, when compared to wild-type cells. Defects in DNA-repair and cell growth, and delayed recruitment of repair proteins to sites of damage were observed. Formation of phosphorylated H2AX and 53BP1 foci was delayed in Mrg15 mutant versus wild-type cells following irradiation. These data implicate a novel role for MRG15 in DNA-damage repair in mammalian cells.

• 出版日期2007-11-13