A new method using Raman spectroscopy for in vivo targeted brain cancer tissue biopsy

作者：Desroches Joannie; Jermyn Michael; Pinto Michael; Picot Fabien; Tremblay Marie Andree; Obaid Sami; Marple Eric; Urmey Kirk; Trudel Dominique; Soulez Gilles; Guiot Marie Christine; Wilson Brian C; Petrecca Kevin; Leblond Frederic

来源：Scientific Reports, 2018, 8(1): 1792.

DOI：10.1038/s41598-018-20233-3

<jats:title>Abstract</jats:title><jats:p>Modern cancer diagnosis requires histological, molecular, and genomic tumor analyses. Tumor sampling is often achieved using a targeted needle biopsy approach. Targeting errors and cancer heterogeneity causing inaccurate sampling are important limitations of this <jats:italic>blind</jats:italic> technique leading to non-diagnostic or poor quality samples, and the need for repeated biopsies pose elevated patient risk. An optical technology that can analyze the molecular nature of the tissue prior to harvesting could improve cancer targeting and mitigate patient risk. Here we report on the design, development, and validation of an <jats:italic>in situ</jats:italic> intraoperative, label-free, cancer detection system based on high wavenumber Raman spectroscopy. This optical detection device was engineered into a commercially available biopsy system allowing tumor analysis prior to tissue harvesting without disrupting workflow. Using a dual validation approach we show that high wavenumber Raman spectroscopy can detect human dense cancer with &gt;60% cancer cells <jats:italic>in situ</jats:italic> during surgery with a sensitivity and specificity of 80% and 90%, respectively. We also demonstrate for the first time the use of this system in a swine brain biopsy model. These studies set the stage for the clinical translation of this optical molecular imaging method for high yield and safe targeted biopsy.</jats:p>

• 出版日期2018-1-29