BSA-protected gold nanoclusters as fluorescent sensor for selective and sensitive detection of pyrophosphate

作者：Liu Jia Ming^*; Cui Ma Lin; Jiang Shu Lian; Wang Xin Xing; Lin Li Ping; Jiao Li; Zhang Li Hong; Zheng Zhi Yong

来源：Analytical Methods, 2013, 5(16): 3942-3947.

DOI：10.1039/c3ay00054k

Gold nanoclusters (AuNCs) protected by bovine serum albumin (BSA) can emit red photoluminescence under illumination by ultraviolet light. The luminescence of the BSA-AuNCs was quenched when Cu2+ chelated with glycine in the BSA chain and generated BSA-AuNCs-Cu2+, while the luminescence of the BSA-AuNCs restored when pyrophosphate ion (P2O74-, PPi) was added and chelated with Cu2+, resulting in Cu2+ being removed from the surface of BSA-AuNCs. Bearing this in mind, herein we present a novel BSA-AuNCs-Cu2+ fluorescent sensor for PPi detection. This environmentally-friendly, simple, rapid and selective fluorescent sensor possesses a wide linear range (0.16-78.1 mu M) and a high sensitivity (the limit of detection (LD) is 0.083 mu M), which could also determine PPi in the effluent of copper plating with the results consistent with those obtained by absorptiometry. Furthermore, the morphologies of BSA-AuNCs, BSA-AuNCs-Cu2+ and BSA-AuNCs-Cu2+-PPi were characterized by high resolution transmission electron microscopy (HRTEM). The mechanism of the proposed assay for PPi detection has been discussed.

• 出版日期2013
• 单位闽南师范大学; 漳州职业技术学院