科研之友
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摘要
A novel resonance Rayleigh scattering (RRS) detection approach combined with high-performance liquid chromatography (HPLC) was developed for the determination of reserpine, deserpidine, and ajmalicine. The resonance Rayleigh scattering signal increased when the analytes were bound to diiodofluorescein in Britton-Robinson buffer (pH 4.2). Separation was performed using a C18 column with a mobile phase consisting of acetonitrile-5 millimolar ammonium acetate buffer at pH 4.2 (44:56, volume by volume). Resonance Rayleigh scattering was measured at excitation and emission wavelengths of 320 nanometers. The experimental parameters affecting the separation and the scattering intensity were carefully optimized. Possible mechanisms for the resonance Rayleigh scattering enhancement of the indole alkaloid-diiodofluorescein system were explored by scanning electron microscopy, nuclear magnetic resonance, and ultraviolet-visible absorption spectroscopy. The method was employed for the determination of the analytes in human urine and pharmaceutical tablets.
- 出版日期2015-6-13
- 单位西南大学
