Short report: Evaluation of direct agglutination test, rk39 test, and ELISA for the diagnosis of visceral leishmaniasis

作者：Mandal Jharna; Khurana Sumeeta; Dubey M L; Bhatia Prateek; Varma Neelam; Malla Nancy^*

来源：The American Journal of Tropical Medicine and Hygiene, 2008, 79(1): 76-78.

The study reports an evaluation of the direct agglutination test (DAT) with use of promastigote/amastigote antigen, rk39 strip test, and ELISA for diagnosis of visceral leishmaniasis (VL). Out of 94 clinically suspected VL patients, 1.6 (17%) were seropositive by all the techniques; in addition 6 were positive in rk39 strip test and ELISA. On retrospective analysis, out of 16 positive by all the techniques, 1.1 (69%) had demonstrable Leishtnania donovani (LD) bodies in their bone marrow samples, while in 5 bone marrow was not examined. Out of 6 that were positive by ELISA and rk39 strip test, 2 had myelofibrosis and 4 had chronic myeloid leukemia. On the basis of bone marrow aspirate positivity, the sensitivity and specificity of DAT were 100% while those of rk39 strip test and ELISA were 100% and 87%, respectively. The study suggests that DAT appears to be the best technique for the serodiagnosis of VL.

• 出版日期2008-7
• 单位河北医科大学