Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets

作者：Pearce Melissa B; Jayaraman Akila; Pappas Claudia; Belser Jessica A; Zeng Hui; Gustin Kortney M; Maines Taronna R; Sun Xiangjie; Raman Rahul; Cox Nancy J; Sasisekharan Ram; Katz Jaqueline M; Tumpey Terrence M^*

来源：Proceedings of the National Academy of Sciences, 2012, 109(10): 3944-3949.

DOI：10.1073/pnas.1119945109

Recent isolation of a novel swine-origin influenza A H3N2 variant virus [A(H3N2)v] from humans in the United States has raised concern over the pandemic potential of these viruses. Here, we analyzed the virulence, transmissibility, and receptor-binding preference of four A(H3N2)v influenza viruses isolated from humans in 2009, 2010, and 2011. High titers of infectious virus were detected in nasal turbinates and nasal wash samples of A(H3N2)v-inoculated ferrets. All four A(H3N2)v viruses possessed the capacity to spread efficiently between cohoused ferrets, and the 2010 and 2011 A(H3N2)v isolates transmitted efficiently to naive ferrets by respiratory droplets. A dose-dependent glycan array analysis of A(H3N2)v showed a predominant binding to alpha 2-6-sialylated glycans, similar to human-adapted influenza A viruses. We further tested the viral replication efficiency of A(H3N2)v viruses in a relevant cell line, Calu-3, derived from human bronchial epithelium. The A(H3N2)v viruses replicated in Calu-3 cells to significantly higher titers compared with five common seasonal H3N2 influenza viruses. These findings suggest that A(H3N2)v viruses have the capacity for efficient replication and transmission in mammals and underscore the need for continued public health surveillance.

• 出版日期2012-3-6