New microsatellite loci for annatto (Bixa orellana), a source of natural dyes from Brazilian Amazonia

作者:Dequigiovanni Gabriel; Ferreyra Ramos Santiago Linorio; Gomes Lopes Maria Teresa; Clement Charles Roland; Rodrigues Doriane Picanco; Fabri Eliane Gomes; Zucchi Maria Imaculada; Veasey Elizabeth Ann
来源:Crop Breeding and Applied Biotechnology, 2018, 18(1): 116-122.
DOI:10.1590/1984-70332018v18n1n18

摘要

Annatto (Bixa orellana) is a tropical crop native to the Americas with Amazonia as the likely center of origin of domestication. Annatto is important because it produces the dye bixin, which is widely used in the pharmaceutical, food, cosmetic and textile industries. A total of 32 microsatellite loci were isolated from a microsatellite- enriched genomic library, of which 12 polymorphic loci were used to characterize four populations of B. orellana and B. orellana var. urucurana, the wild relative. Higher genetic diversity estimates were detected for the wild populations when compared to the cultivated populations. Also, higher apparent outcrossing rates were found for the two wild than the cultivated populations. These results indicate a mixed mating system for the species. All markers described herein have potential to be used in further studies evaluating the genetic diversity, population dynamics, domestication, breeding, and conservation genetics of annatto.

  • 出版日期2018-3