科研之友
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摘要
In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is developed for determination of voriconazole (VRC) in human plasma samples using lorazepam as the internal standard (IS). Sample preparation was accomplished through one-step protein precipitation with acetonitrile, and chromatographic separation was carried out on an Agilent ZORBAX ExtendC18 (4.6 x 250 mm, 5 m) at 50 degrees C. Mobile phase composed of a mixture of acetonitrile-0.15 M NaH2PO4-water (45:20:35) at flow rate of 0.9 mL/min. Wavelength was set at 256 nm. The chromatographic retention times of VRC and IS were 6.4 and 5.6 min, respectively. The lower limit of quantitation (LLOQ) was 5 ng/mL, and no interferences were detected in the chromatograms. The devised HPLC method was validated by evaluating its intra-and inter-day precisions and accuracies in a linear concentration range between 5 and 1500 ng/mL. The method was successfully applied to a pharmacokinetic study of oral VRC tables in Chinese healthy volunteers.
