Association Analysis between the Polymorphism of the SLC11A1 Gene and Immune Response Traits in Pigs

作者：Liu Yang; Qiu Xiaotian; Xu Jingjing; Hu Fang; Li Yuhua; Li Hejun; Gong Yuanfang; Zhang Qin^*

来源：Asian Journal of Animal and Veterinary Advances, 2011, 6(6): 580-586.

DOI：10.3923/ajava.2011.580.586

• SLC11A1
• polymorphism
• PCR-RFLP
• pig
• immune traits
• association analysis

Solute carrier family 11 member 1 (SLC11A1) plays an important role in the innate immune response against intracellular pathogens. To evaluate effect of SLC11A1 gene on immune response capacity in pigs, one SNP in intron 1 was demonstrated by PCR-RFLP and sequencing method. Immune response traits include six normal hematology traits (white blood cell, red blood cell, hemoglobin, granulocyte, lymphocyte and monocyte), four T lymphocyte subpopulations (CD4(-)CD8(-), CD4(-)CD8(+), CD4(+)CD8(-), CD4(+)CD8(+)) and CSFV antibody titer were measured in three pig breeds (Large White, Landrace and Songliao Black) after vaccinated with classical swine fever live vaccine at the 21st day after birth. The further association analysis between the SNP genotype and immune response traits were conducted. The SNP of SLC11A1 gene had significant effect on level of monocyte (p = 0.010) and CD4(-)CD8(+) percentage (p = 0.041). The animals with AA genotype had significant higher monocyte and CD4(-)CD8(+) percentage than that of animals with GG and GA genotype (p<0.05). Present results also suggest that SLC11A1 could be a marker gene for genetic selection of disease susceptibility in pigs.

• 出版日期2011-6
• 单位中国农业大学