Lymphocytes eject interferogenic mitochondrial DNA webs in response to CpG and non-CpG oligodeoxynucleotides of class C

作者：Ingelsson Bjoern; Soderberg Daniel; Strid Tobias; Soderberg Anita; Bergh Ann Charlotte; Loitto Vesa; Lotfi Kourosh; Segelmark Marten; Spyrou Giannis; Rosen Anders^*

来源：Proceedings of the National Academy of Sciences, 2018, 115(3): E478-E487.

DOI：10.1073/pnas.1711950115

• mitochondrial DNA release
• immune DNA sensing
• lymphocyte signaling
• DAMP
• CpG-C

Circulating mitochondrial DNA (mtDNA) is receiving increasing attention as a danger-associated molecular pattern in conditions such as autoimmunity, cancer, and trauma. We report here that human lymphocytes [B cells, T cells, natural killer (NK) cells], monocytes, and neutrophils derived from healthy blood donors, as well as B cells from chronic lymphocytic leukemia patients, rapidly eject mtDNA as web filament structures upon recognition of CpG and non-CpG oligodeoxynucleotides of class C. The release was quenched by ZnCl2, independent of cell death (apoptosis, necrosis, necroptosis, autophagy), and continued in the presence of TLR9 signaling inhibitors. B-cell mtDNA webs were distinct from neutrophil extracellular traps concerning structure, reactive oxygen species (ROS) dependence, and were devoid of antibacterial proteins. mtDNA webs acted as rapid (within minutes) messengers, priming antiviral type I IFN production. In summary, our findings point at a previously unrecognized role for lymphocytes in antimicrobial defense, utilizing mtDNA webs as signals in synergy with cytokines and natural antibodies, and cast light on the interplay between mitochondria and the immune system.

• 出版日期2018-1-16