Aging sensitizes rapidly isolated hippocampal microglia to LPS ex vivo

作者：Frank Matthew G^*; Barrientos Ruth M; Watkins Linda R; Maier Steven F

来源：Journal of Neuroimmunology, 2010, 226(1-2): 181-184.

DOI：10.1016/j.jneuroim.2010.05.022

• Aging
• Neuroinflammation
• Sensitization
• Priming
• Pro-inflammatory cytokines
• Microglia

The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1 beta and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.

• 出版日期2010-9-14