Antiviral and antiproliferative effects of canine interferon-lambda 1

作者：Ichihashi Tomonori; Asano Atsushi^*; Usui Tatsufumi; Takeuchi Takashi; Watanabe Yasuko; Yamano Yoshiaki

来源：Veterinary Immunology and Immunopathology, 2013, 156(1-2): 141-146.

DOI：10.1016/j.vetimm.2013.09.013

• Antiviral activity
• Antiproliferation
• Dog
• Interferon-stimulated genes
• IFN-lambda 1

Interferon (IFN)-lambda s, members of the type III IFN group, were recently identified in several vertebrates. Although IFN-lambda s have the potential to be utilized as antiviral and antitumor agents in veterinary medicine, the biological properties of IFN-lambda s have not yet been studied in companion animals. In this study, we analyzed the expression of canine IFN-lambda s and their receptors, produced a recombinant canine IFN-lambda 1 protein, and investigated its antiviral and antiproliferative activities using a canine kidney epithelial cell line, MDCK cells. MDCK cells were found to express type III IFN molecules, IFN-lambda 1 and IFN-lambda 3, and the receptors, IFN lambda R1 and IL10R2. IFN-lambda 1 was induced faster than IFN-lambda 3 by stimulation with poly (I:C). His-tagged IFN-lambda 1 protein expressed in Escherichia coli inhibited cytolytic plaque formation by influenza A virus infection, and induced the expression of interferon-stimulated genes, Mx1 and OAS1, in MDCK cells. In addition, recombinant IFN-lambda 1 inhibited the proliferation of MDCK cells slightly. These effects were observed in a dose-dependent manner. These results indicate that canine IFN-lambda 1 has antiviral effect, and suggest the potential applicability of canine IFN-lambda 1 as a therapeutic agent.

• 出版日期2013-11-15