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摘要
The effects of previous cold-induced cell elongation on adherence of Escherichia coli O157:H7 to glass slides and stainless steel surfaces was evaluated at 4 degrees C for %26lt;= 48 h. Planktonic E. coli O157:H7 with and without cold adaptation were prepared at 15 and 37 degrees C, respectively, and planktonic E. coli O157:H7 containing elongated (%26gt; 4 %26lt;= 10 mu m) and filamentous (%26gt; 10 mu m) cells were prepared at 6 degrees C. Despite morphological differences in planktonic E. coli O157:H7 preparations, all three cell types attached to a greater extent to glass than to the stainless steel surfaces. E. coli O157:H7 cells adapted to growth at 15 degrees C attached better to both glass and stainless steel surfaces (3.2 and 2.6 log cfu/cm(2), respectively) than cells of the other treatments at %26gt;= 24 h. Cells adapted at 6 degrees C attached to glass slides and stainless steel coupons at levels of 3.0 and 1.8 log cfu/cm(2), respectively, while E. coli O157:H7 cells grown at 37 degrees C attached to these surfaces at levels of 2.0 and 1.7 log cfu/cm(2), respectively. No further attachment of cells from any of the treatments was noted between 24 and 48 h at 4 degrees C. These results suggest that E. coli O157:H7 cells adapted at 6 degrees C-15 degrees C have greater potential to attach to food contact surfaces than those grown at higher temperature. The enhanced biofilm-forming ability of 6 degrees C or 15 degrees C-adapted, elongated and filamentous E. coli O157:H7 cells did not appear to be related to the greater entanglement of longer cells within biofilm matrices.
- 出版日期2013-4
