An animal model of atherosclerotic plaque disruption and thrombosis in rabbit using pharmacological triggering to plaques induced by perivascular collar placement

作者：Sun, Xin; Cao, Wei; Cui, Jinjin; Wang, Liping; Ma, Lansi; Wang, Tengyu; Peng, Chenghai; Tian, Zhen; Shi, Sa; Guo, Shuyuan; Tian, Ye^*

来源：Cardiovascular Pathology, 2013, 22(4): 264-269.

DOI：10.1016/j.carpath.2012.11.003

• Atherosclerosis
• Thrombosis
• Silastic collar
• Russell's viper venom
• Histamine

Introduction: Limited availability of suitable animal model of plaque disruption and thrombosis has hampered the study of mechanism and preclinical evaluation of plaque-stabilizing therapies. This study aims to develop an animal model of atherosclerotic plaque disruption and thrombosis in rabbit femoral artery. @@@ Methods: Silastic collars were placed around the bilateral femoral arteries of rabbits, which had been fed with atherogenic diet for 7 days. After 28 days on the same diet, the rabbits received pharmacological triggering by intraperitoneal injection of Russell's viper venom (RVV, 0.15 mg/kg) followed by intravenous injection of histamine (0.02 mg/kg), and the animals were then processed for imageological and histological examinations. @@@ Results: Perivascular collar placement of the femoral artery in high-cholesterol-fed rabbits for 28 days induced marked intimal hyperplasia, which was a lipid- and collagen-rich lesion that contained substantial amount of macrophages and smooth muscle cells. Subsequent histological analysis showed that the pharmacological triggering evoked plaque disruption and platelet- and fibrin-rich thrombi in the collared femoral arteries. @@@ Conclusion: We demonstrated, for the first time, a rabbit model of plaque disruption and thrombosis induced by the combination of perivascular collar placement, RVV, and histamine injections. This model can be rapidly formed, easily operated, and site controlled.

• 出版日期2013-7
• 单位哈尔滨医科大学