MicroRNA-145 Function as a Cell Growth Repressor by Directly Targeting c-Myc in Human Ovarian Cancer

作者：Zhang, Wenjing; Wang, Qian; Yu, Minglian; Wu, Nan; Wang, Hui^*

来源：Technology in Cancer Research and Treatment, 2014, 13(2): 161-168.

DOI：10.7785/tcrt.2012.500367

• MiR-145
• c-Myc
• Ovarian cancer
• Proliferation
• Apoptosis

MiR-145 is reported to be significantly down-regulated in ovarian cancer. This study was aimed at elucidating the roles of miR-145 in regulating the biological behavior of epithelial ovarian cancer (EOC) cells. In this report, we find out that up-regulation of miR-145 in OVCAR-3 and SKOV-3 cells inhibit cell proliferation and promote cell apoptosis. We show that miR-145 directly target the c-Myc 3'-UTR. Moreover, ectopic expression of c-Myc reduces the inhibition of cell proliferation caused by miR-145 transfection. Cell cycle assay showed that up-regulation of miR-145 reduces S phase population, and restoration of c-Myc can rescue this reduction. These findings indicate that miR-145 inhibits cell proliferation and promotes cell apoptosis by targeting c-Myc 3'-UTR. Therefore, the result indicated that miR-145 could be used as a potential therapeutic target in ovarian cancer.

• 出版日期2014-4
• 单位中国人民解放军陆军总医院