NAC ameliorates dental composite-induced DNA double-strand breaks and chromatin condensation

作者：Styllou Panorea^*; Styllou Marianthi; Nickel Reinhard; Hogg Christof; Reichl Franz Xaver; Scherthan Harry

来源：Dental Materials Journal, 2017, 36(5): 638-646.

DOI：10.4012/dmj.2016-316

• NAC
• DNA damage
• gamma-H2AX
• 53BP1
• Chromatin

Released (co)monomers from dental composite components can induce DNA damage of which DNA double-strand breaks (DSBs) threaten genome integrity. Here, we tested whether the administration of the antioxidant N-acetylcysteine (NAC) is able to reduce the dental composite-induced DSBs in primary human gingiva fibroblasts. The dental composites Bis-GMA (bisphenol-Aglycerolate dimethacrylate), GlVIA (glycidyl methacrylate), HEMA (2-hydroxyethyl methacrylate) and TEGDMA (triethyleneglycol dimethacrylate) were found to induce co-localizing microscopic nuclear foci numbers of the DSB markers gamma-H2AX and 53BP1 per cell in the order: GMA>Bis-GMA>TEGDMA>HEMA. Supplementation of (co)monomer-containing culture medium with NAC led to a significant reduction of resin-induced DSBs as well as to an amelioration of dental monomer-induced nuclear chromatin condensation in gingival fibroblasts. Thus, antioxidant treatment can reduce radical-induced chromatin and DNA damage and open avenues to mitigate genotoxic effects of dental composite compounds.

• 出版日期2017-10