Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A

作者：Chen Guanqun; Greer Michael S; Lager Ida; Yilmaz Jenny Lindberg; Mietkiewska Elzbieta; Carlsson Anders S; Stymne Sten; Weselake Randall J^*

来源：FEBS LETTERS, 2012, 586(4): 373-377.

DOI：10.1016/j.febslet.2011.12.034

• Hydrolase
• Phospholipase A
• Arabidopsis thaliana
• Saccharomyces cerevisiae

A previously uncharacterized Arabidopsis lecithin: cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine %26gt; phosphatidic acid %26gt; phosphatidylethanolamine %26gt; phosphatidylglycerol %26gt; phosphatidylserine and has an acyl chain specificity for oleoyl %26gt; linoleoyl %26gt; ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.

• 出版日期2012-2-17