A Fok I/DNA Machine that Duplicates its Analyte Gene Sequence

作者：Weizmann Yossi; Cheglakov Zoya; Willner Itamar^*

来源：Journal of the American Chemical Society, 2008, 130(51): 17224-+.

DOI：10.1021/ja806222e

A method for the amplified analysis of a gene sequence by a Fok I/DNA machine is described. A tailored nucleic acid that includes a recognition sequence for the analyzed gene and a hybridized sequence of the gene itself acting as the "fuel" substrate of the "machine". Upon recognition of the gene by the "fuel" substrate, the autonomous Fok I-induced cleavage of the resulting duplex proceeds, resulting in the duplication of the gene sequence and the horseradish peroxidase mimicking hemin/G-quadroplex DNAzyme as a chemiluminescence reporter. The autonomous duplication of the gene sequence and the catalytic activity of the DNAzyme provide a double amplification path that enables the analysis of target DNA with a detection limit of 1 x 10(-14) M.

• 出版日期2008-12-24