Allicin disrupts the cell's electrochemical potential and induces apoptosis in yeast

作者：Gruhlke Martin C H; Portz Daniela; Stitz Michael; Anwar Awais; Schneider Thomas; Jacob Claus; Schlaich Nikolaus L; Slusarenko Alan J^*

来源：Free Radical Biology and Medicine, 2010, 49(12): 1916-1924.

DOI：10.1016/j.freeradbiomed.2010.09.019

• Redox
• Glutathione
• Garlic
• Allium
• Free radicals

The volatile substance allicin gives crushed garlic (Allium sativum) its characteristic odor and is a pro-oxidant that undergoes thiol-disulfide exchange reactions with -SH groups in proteins and glutathione The antimicrobial activity of allicin is suspected to be due to the oxidative inactivation of essential thiol-containing enzymes We investigated the hypothesis that at threshold inhibitory levels allicin can shunt yeast cells Into apoptosis by altering their overall redox status Yeast cells were treated either with chemically synthesized pure allician or with allicin in garlic juice Allicin-dependent cell oxidation was demonstrated with a redox-sensitive GFP construct and the shift in cellular electrochemical potential (E-hc) from less than -215 to -181 mV was calculated using the Nernst equation after the glutathione/glutathione disulfide couple (2GSH/GSSG) in the cell was quantified Caspase activation occurred after allicin treatment and yeast expressing a human antiapoptotic Bcl-XL construct was rendered more resistant to allicin Also a yeast apoptosis-inducing factor deletion mutant was more resistant to anion than wild-type cells We conclude that allicin in garlic juice can activate apoptosis in yeast cells through its oxi

• 出版日期2010-12-15