Toll-Like Receptor 2-Dependent Extracellular Signal-Regulated Kinase Signaling in Mycobacterium tuberculosis- Infected Macrophages Drives Anti-Inflammatory Responses and Inhibits Th1 Polarization of Responding T Cells

作者：Richardson Edward T; Shukla Supriya; Sweet David R; Wearsch Pamela A; Tsichlis Philip N; Boom W Henry; Harding Clifford V^*

来源：Infection and Immunity, 2015, 83(6): 2242-2254.

DOI：10.1128/IAI.00135-15

Mycobacterium tuberculosis survives within macrophages and employs immune evasion mechanisms to persist in the host. Protective T helper type 1 (Th1) responses are induced, and the immune response in most individuals is sufficient to restrict Mycobacterium tuberculosis to latent infection, but most infections are not completely resolved. As T cells and macrophages respond, a balance is established between protective Th1-associated and other proinflammatory cytokines, such as interleukin-12 (IL-12), interferon gamma (IFN-gamma), and tumor necrosis factor alpha, and anti-inflammatory cytokines, such as IL-10. The mechanisms by which Mycobacterium tuberculosis modulates host responses to promote its survival remain unclear. In these studies, we demonstrate that Mycobacterium tuberculosis induction of IL-10, suppression of IL-12, and inhibition of class II major histocompatibility complex (MHC-II) molecules in infected macrophages are all driven by Toll-like receptor 2 (TLR2)-dependent activation of the extracellular signal-regulated kinases (ERK). Elimination of ERK signaling downstream of TLR2 by pharmacologic inhibition with U0126 or genetic deletion of Tpl2 blocks IL-10 secretion and enhances IL-12 p70 secretion. We demonstrate that Mycobacterium tuberculosis regulation of these pathways in macrophages affects T cell responses to infected macrophages. Thus, genetic blockade of the ERK pathway in Tpl2(-/-) macrophages enhances Th1 polarization and IFN-gamma production by antigen-specific CD4(+) T cells responding to Mycobacterium tuberculosis infection. These data indicate that Mycobacterium tuberculosis and its potent TLR2 ligands activate ERK signaling in macrophages to promote anti-inflammatory macrophage responses and blunt Th1 responses against the pathogen.

• 出版日期2015-6