Antagonistic effect of TNF-alpha and insulin on uncoupling protein 2 (UCP-2) expression and vascular damage

作者：Gomez Hernandez Almudena^*; Perdomo Liliana; de las Heras Natalia; Beneit Nuria; Escribano Oscar; Otero Yolanda F; Guillen Carlos; Diaz Castroverde Sabela; Gozalbo Lopez Beatriz; Cachofeiro Victoria; Lahera Vicente; Benito Manuel

来源：Cardiovascular Diabetology, 2014, 13(1): 108.

DOI：10.1186/s12933-014-0108-9

• TNF-alpha
• Insulin
• UCP-2
• Atherogenesis

Background: It has been reported that increased expression of UCP-2 in the vasculature may prevent the development of atherosclerosis in patients with increased production of reactive oxygen species, as in the diabetes, obesity or hypertension. Thus, a greater understanding in the modulation of UCP-2 could improve the atherosclerotic process. However, the effect of TNF-alpha or insulin modulating UCP-2 in the vascular wall is completely unknown. In this context, we propose to study new molecular mechanisms that help to explain whether the moderate hyperinsulinemia or lowering TNF-alpha levels might have a protective role against vascular damage mediated by UCP-2 expression levels.
Methods: We analyzed the effect of insulin or oleic acid in presence or not of TNF-alpha on UCP-2 expression in murine endothelial and vascular smooth muscle cells. At this step, we wondered if some mechanisms studied in vitro could be of any relevance in vivo. We used the following experimental models: ApoE(-/-) mice under Western type diet for 2, 6, 12 or 18 weeks, BATIRKO mice under high-fat diet for 16 weeks and 52-week-old BATIRKO mice with o without anti-TNF-alpha antibody pre-treatment.
Results: Firstly, we found that TNF-alpha pre-treatment reduced UCP-2 expression induced by insulin in vascular cells. Secondly, we observed a progressive reduction of UCP-2 levels together with an increase of lipid depots and lesion area in aorta from ApoE(-/-) mice. In vivo, we also observed that moderate hyperinsulinemic obese BATIRKO mice have lower TNF-alpha and ROS levels and increased UCP-2 expression levels within the aorta, lower lipid accumulation, vascular dysfunction and macrovascular damage. We also observed that the anti-TNF-alpha antibody pre-treatment impaired the loss of UCP-2 expression within the aorta and relieved vascular damage observed in 52-week-old BATIRKO mice. Finally, we observed that the pretreatment with iNOS inhibitor prevented UCP-2 reduction induced by TNF-alpha in vascular cells. Moreover, iNOS levels are augmented in aorta from mice with lower UCP-2 levels and higher TNF-alpha levels.
Conclusions: Our data suggest that moderate hyperinsulinemia in response to insulin resistance or lowering of TNF-a levels within the aorta attenuates vascular damage, this protective effect being mediated by UCP-2 expression levels through iNOS.

• 出版日期2014-7-31