A Non-enzyme Amperometric Immunosensor for Rapid Determination of Human Immunodeficiency Virus p24 Based on Magnetism Controlled Carbon Nanotubes Modified Printed Electrode

作者：Gan Ning; Luo Nai Xin; Li Tian Hua; Zheng Lei; Ni Min Jun

来源：Chinese Journal of Analytical Chemistry, 2010, 38(11): 1556-1562.

DOI：10.3724/SP.J.1096.2010.01556

• N,N '-Bis (2-hydroxy-methylene)-o-phenylenediamine copper
• Screen printed carbon electrode
• Gold magnetic particles/carbon nanotube composite particles
• Human immunocleficency virus capsid protein 24
• Magnetic non-enzyme immunosensor

A magnetism controlled and non-enzyme amperometric immunosensor was fabricated for the determination of human immunodeficiency virus p24 (HIV p24). First, Fe(3)O(4)/Au composite nanoparticles (GMP) were modified on multiwalled carbon nanotubes (MWNTs-GMP). Then, antibody of p24(anti p24) was coated on it to prepare MWNTs-GMP/anti p24 biomarker. Finally, the marker was absorbed on the surface of N,N'-Bis (2-hydroxy-methylene)-o-phenylenediamine copper (CuRb) modified screen-printed carbon electrodes (SPCEs) through external magnetic field in which CuRb can be employed as an electron transfer mediator and catalyst for detection of H(2)O(2). After the immunosensor is incubated with p24 sample at room temperature for 15 min, the electron transfer access of CuRb to H(2)O(2) is partly inhibited, which leads to a linear decrease of the catalytic efficiency to H(2)O(2) at 300 mV in pH 7.0 PBS. Under optimal conditions, the linear range of p24 is from 0.6 to 160 mu g/L and the detection limit is 0.32 mu g/L at 3 times noise. The immunosensor was employed to determine p24 in acquired immure deficiency syndrome (AIDs) patients' serum samples and the results were consistent to the tradition ELISA method which was suitable for screen determination of trace p24 in serums of AIDs' patients.

• 出版日期2010-11
• 单位宁波大学