Determination of Myricetin in Rat and Rabbit Plasma by UPLC-MS/MS and Application to Pharmacokinetic Study

作者：Cai Xiao jun; Yang Qiu jie; Chen Da hui; Hu Shu ping; Zheng Jing; Shen Jia qi; Xiang Zheng^*

来源：Latin American Journal of Pharmacy, 2015, 34(7): 1373-1379.

• Myricetin
• UPLC-MS/MS
• Pharmacokinetics

Myricetin is a member of the flavonoid class of polyphenolic compounds, with antioxidant properties. An ultra-performance liquid chromatography with tandem mass spectrometry (UPLC - MS/MS) method was established for the determination of myricetin in rat and-rabbit plasma. Myricetin was administered by sublingual intravenous and ear-vein in rats and rabbits, respectively. The plasma samples from the rats and rabbits were extracted by ethyl acetate. Chromatographic separation was achieved on a HSS T3 column and the analytes were detected by using electrospray ionization (ESI) source with a positive ion mode. The pharmacokinetic parameters were analyzed by drug and statistic software (DAS). The result showed the good linearity in the concentration range of 1-1000 ng/mL for rat and 5-1000 ng/mL for rabbit, respectively. The intra- and inter-day precision was within 7.8 % and the accuracy ranged from 85.0 % to 91.1 %. The simple, sensitive and rapid UPLC-MS/MS method was successfully applied to the pharmacokinetic study of myricetin in rat and rabbit.

• 出版日期2015-9
• 单位温州医科大学