Differences in Cell Wall Thickness between Resistant and Nonresistant Strains of Mycobacterium tuberculosis: Using Transmission Electron Microscopy

作者：Velayati Ali Akbar; Farnia Parissa; Ibrahim Tengku Azmi; Haroun Rafiuz Zaman; Kuan Ho Oi; Ghanavi Jalaledin; Farnia Poopak; Kabarei Ali Naghee; Tabarsi Payam; Omar Abdul Rahman; Varahram Mohammad; Masjedi Mohammad Reza

来源：Chemotherapy, 2009, 55(5): 303-307.

DOI：10.1159/000226425

• Tuberculosis
• Cell wall
• TEM

The ultrastructure of the cell wall of extensively drug-resistant (XDR), multidrug-resistant (MDR) and susceptible tuberculosis ( TB) bacilli was viewed under transmission electron microscopy (TEM). Under the TEM, marked differences were observed in the thickness of their cell wall: 20.2 /- 1.5 and 17.1 /- 1.03 nm for the XDR and MDR TB bacilli, respectively, and 15.6 /- 1.3 nm for the susceptible isolates (p < 0.05). In MDR bacilli, thickening of the cell wall was observed in the intermediate electron-transparent layer (ETL) and outer electron-opaque layer, whereas in XDR TB cells the basal peptidoglycan layer was denser and almost fused with the ETL. Five to seven percent of XDR TB bacilli had the appearance of the stationary phase with cell wall thickness ranging from 21 to 26 nm (p < 0.001). Information provided in this study is of significant importance in terms of drug selection for effective treatment of resistant strains.

• 出版日期2009