A Single-Cell Sequencing Guide for Immunologists

作者：See, Peter; Lum, Josephine; Chen, Jinmiao^*; Ginhoux, Florent^*

来源：Frontiers in Immunology, 2018, 9: 2425.

DOI：10.3389/fimmu.2018.02425

• single-cell RNA sequencing
• MARS-seq
• SMART-seq
• fluidigm C1
• 10X genomics chromium
• immunology
• dendritic cells

In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) approaches in the field of immunology. With the wide range of technologies available, it is becoming harder for users to select the best scRNA-seq protocol/platform to address their biological questions of interest. Here, we compared the advantages and limitations of four commonly used scRNA-seq platforms in order to clarify their suitability for different experimental applications. We also address how the datasets generated by different scRNA-seq platforms can be integrated, and how to identify unknown populations of single cells using unbiased bioinformatics methods.

• 出版日期2018-10-23
• 单位上海交通大学